New Era, New Criteria for City Imaging

Despite many dramatic changes in last fifty years, urban designers still try to measure "city imaging" with same criteria as was described by Kevin Lynch in his landmark volumes. However, city imaging increasingly is supplemented by exposure to recent progresses in communication infrastructures. It seems that once again we need to re-ontologize concepts of city imaging for twenty-first century as his ideas were theoretically created when digital evolution was not such widely affected our world. This paper aims to identify effects of these new digital actors on city imaging. Consequently, we propose that with emergence of Softerial Era and infospherization of almost everything, another kind of landmarks have been evolved (linkmarks) which are highly referenced to a self and relative to his destination and necessities in real-time. We believe they can improve not only our “sense of place” but also “sense of time” as an inevitable necessity in our current life.Kevin Lynch, City Imaging, Infospherization, Landmark, Sense of time.

ANALYZING WORLD EVOLUTION AND ITS EFFECTS ON URBAN DESIGNING

Our society's modalities of communication and hence our cities have been rapidly changed due to emergence of several revolutions most lately the digital one. In fact, recently with emergence of the fluid, responsive, kinetic, data-driven worlds of infoscape and its combination with urban landscape, urban designing faces a radical reshuffling of a number of its principal underpinnings. It seems that once again we as urban designers in order to catch up with the current world's situation need to evolve or in better words to re-ontologize concepts of urban designing for twenty-first century. Therefore; in this paper for better understanding of the main characteristics of current changes we try to identify the effects of new actors on urban structures trough analysis of different evolution phases of our cities. To do so we make a diagram called "Evolutionary Trend" trough which we can trace world's evolution history to help us know where we are and what may happen in coming decades. This "evolutionary trend" can be used as a guideline for urban designers to help them navigate better in future.Evolutionary Trend, Urban Evolution, Infospherization, Softerial Era, Digital revolution

青年期学生の疲労自覚症状に関する研究

取得学位:博士（学術）,授与番号:博士甲第22号,授与年月日:1999年3月22日,授与大学:金沢大学,論文審査委員長:出村, 愼一, 論文審査委員:　安川, 哲夫 / 喜多尾, 浩

Comprehensive Evaluation of Selected Methods for Assessing Human Body Composition

The present study investigated the validity, reliability, objectivity, and practicability of methods with skinfold caliper (SF), ultrasound (US), bioelectrical impedance (BI), and sulfur hexafluoride (SHD) using identical subjects, and evaluated comprehensive usefulness by comparing selected methods or equations. In examining validity, underwater weighing (UW) was employed to obtain the criterion of validity. The subjects were healthy Japanese, 16 males and 15 females, aged 18 to 32 years. The prediction equation developed by Nakadomo et al. (1990b) was considered to be suitable for BI. With respect to the validity, BI and SHD assessing total body when estimating body composition would be more valid than those assessing partial subcutaneous skinfold thickness. A comprehensive evaluation through an examination with respect to the validity, reliability, objectivity, and practicability suggests that the BI could be the best method to assess human body composition in vivo

Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

<p>Abstract</p> <p>Background</p> <p>Various protein expression systems, such as <it>Escherichia coli </it>(<it>E. coli</it>), <it>Saccharomyces cerevisiae </it>(<it>S. cerevisiae</it>), <it>Pichia pastoris </it>(<it>P. pastoris</it>), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled receptors (GPCRs) for structural studies. Recently, the crystal structures of four recombinant human GPCRs, namely β₂adrenergic receptor, adenosine A_2areceptor, CXCR4 and dopamine D3 receptor, were successfully determined using an insect cell expression system. GPCRs expressed in insect cells are believed to undergo mammalian-like posttranscriptional modifications and have similar functional properties than in mammals. Crystal structures of GPCRs have not yet been solved using yeast expression systems. In the present study, <it>P. pastoris </it>and insect cell expression systems for the human muscarinic acetylcholine receptor M2 subtype (CHRM2) were developed and the quantity and quality of CHRM2 synthesized by both expression systems were compared for the application in structural studies.</p> <p>Results</p> <p>The ideal conditions for the expression of CHRM2 in <it>P. pastoris </it>were 60 hr at 20°C in a buffer of pH 7.0. The specific activity of the expressed CHRM2 was 28.9 pmol/mg of membrane protein as determined by binding assays using [³H]-quinuclidinyl benzilate (QNB). Although the specific activity of the protein produced by <it>P. pastoris </it>was lower than that of Sf9 insect cells, CHRM2 yield in <it>P. pastoris </it>was 2-fold higher than in Sf9 insect cells because <it>P. pastoris </it>was cultured at high cell density. The dissociation constant (Kd) for QNB in <it>P. pastoris </it>was 101.14 ± 15.07 pM, which was similar to that in Sf9 insect cells (86.23 ± 8.57 pM). There were no differences in the binding affinity of CHRM2 for QNB between <it>P. pastoris </it>and Sf9 insect cells.</p> <p>Conclusion</p> <p>Compared to insect cells, <it>P. pastoris </it>is easier to handle, can be grown at lower cost, and can be expressed quicker at a large scale. Yeast, <it>P. pastoris</it>, and insect cells are all effective expression systems for GPCRs. The results of the present study strongly suggested that protein expression in <it>P. pastoris </it>can be applied to the structural and biochemical studies of GPCRs.</p

Roles of SiH4 in growth, structural changes and optical properties of nanocrystalline silicon thin films

金沢大学理工研究域電子情報通信学系Nanocrystalline silicon (ns-Si) thin films deposited through plasma-enhanced chemical vapor deposition technique were studied. These films were grown at low deposition temperature of 200°C and at different silane flow rates ([SiH4]). Characterization of these films with Raman spectroscopy, x-ray diffraction and atomic force microscopy revealed that no films deposited at [SiH4]=0.0sccm. In addition, the structural change from an amorphous to a nanocrystalline phase at [SiH4]=0.2sccm. The Fourier transform infrared spectroscopic analysis showed at low values of [SiH4](0.1sccm), no hydrogen incorporated in the nc-Si thin film. However, the intensity of the spectra around 2100 cm-1 is likely to decreases with increasing [SiH4]. We have observed photoluminescence (PL) at room temperature in the range of 1.7 eV to 2.4 eV for all the films. Presence of the very small crystallites (the size less than 20 nm) responsible for quantum confinement effect. Variations of the PL intensity, width and position are well correlation with the structural properties of the films such as crystalline size, crystalline volume fraction, and hydrogen content. Furthermore, the PL emissions also showed correlation with the distribution of spherical grains with the size below 50 nm distributed on the films surface. © 2011 American Institute of Physics.Conference Pape

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead